ELISA Flex: Mouse IFN-α (HRP)

ELISA Flex: Mouse IFN‑α (HRP)

3326-1H-6

Publications: 1
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT24A/44A/104
Detection mAbMT9L/14A/113, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant mouse IFN-α ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

Shipping $0

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ELISA substrate: TMB for HRP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IFN-α in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Mouse IFN-α (HRP)
ApplicationELISA
AnalyteIFN-α pan
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of mouse IFN-α (pan specific). Subtypes 1, 2, 4, 5, 6T, 7/10 (129/Sv), 8/6, 9, 11, 12, 13, 14, 15 (A), and B are detected.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard range8-1000 pg/ml
Representative standard curve for mouse IFN-α pan ELISA Flex kit using TMB substrate.
Datasheet/Protocol
SDS

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

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Behind the scenes, Published March 27, 2023

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IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells. 

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative namesInterferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA